Veterinary and Comparative Biomedical Research

Veterinary and Comparative Biomedical Research

The Effect of Liposome Nanocarrier Containing 2% Arnebia euchroma Extract on Infectious Burn Wound Healing in Rats

Articles in Press

Document Type : Original Article

Authors
Mohammadmahdi Alinaghizadeh 1
Mohammad Mahdi Molaei 1
Ehsanollah Sakhaee 1
Reza Kheirandish 2
Pourya Mohammadi 3
Fatemeh Heydari 1
Reza Molaei 2
Fateme Mohebbi Pourkani 1
1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
2 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Research group of Tropical infectious diseases, Kerman, Iran
3 Department of Chemistry, Shahid Bahonar University of Kerman, Kerman, Iran
10.22103/VCBR.2025.25132.1064
Abstract
This study investigated the effect of a hyaluronic acid-based hydrogel containing 2% Arnebia Euchroma extract-loaded nanoliposomes on the healing of second-degree burn wounds infected with Pseudomonas aeruginosa in rats. Twenty-four male Wistar albino rats (48 wounds total) were randomly divided into six groups of eight. Second-degree burns were induced on the right and left flanks of each rat using a 1 cm diameter, 100-gram copper rod heated to 100°C. The wounds were then infected with Pseudomonas aeruginosa and covered with a dressing for 48 hours. Treatment commenced on subsequent days. On days 9 and 15, half of the animals from each group were euthanized, and skin samples were collected. Histopathological parameters, including inflammation, granulation tissue formation, collagen content, epidermal regeneration, and angiogenesis, were evaluated. Evaluation of wound healing at days 9 and 15 revealed that the positive control group and the standard treatment group exhibited the greatest improvement compared to other groups. However, the group treated with Hydroalcoholic extract of Arnebia Euchroma displayed a different healing pattern. On day 9, this group outperformed other groups in some evaluated parameters, but on day 15, wound healing in this group was slower. These findings suggest that the hyaluronic acid hydrogel containing Arnebia Euchroma extract-loaded nanoliposomes has significant potential for accelerating the healing of superficial infected burn wounds.
Keywords
Arnebia euchroma
Infectious Wound Healing
Rat
Histopathology
Nano-Liposomes
Hyaluronic acid
Subjects
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Veterinary and Comparative Biomedical Research

Articles in Press, Accepted Manuscript
Available Online from 11 July 2026