Relationship between Follicle Wall Diameter, Secretory Activity, and Histopathological Characteristics of Cyst-like Structures in Bovine Ovaries: An Abattoir Survey

Document Type : Original Article

Authors

1 School of Veterinary Medicine, Lorestan University, Khorram Abad, Iran

2 Department of Clinical Sciences, School of Veterinary Medicine, Lorestan University, Khorram Abad, Iran

3 Department of Pathobiology, School of Veterinary Medicine, Lorestan University, Khorram Abad, Iran

4 Department of Basic Sciences, School of Veterinary Medicine, Lorestan University, Khorram Abad, Iran

Abstract

Ovarian cystic follicles are one of the most important reproductive problems in cows and animal husbandry. The aim of this study was to evaluate the relationship between follicle wall diameter and secretory and histopathological characteristics of cyst-like structure in the diagnosis of the type of ovarian cysts in slaughtered cows. In this study, 28 follicles larger than 25 mm in diameter and eight samples of follicles smaller than 20 mm in diameter were examined. Based on the criteria of follicular wall diameter, the samples were classified into three groups: control (non-cystic), follicular, and luteal groups. Pathological sections were prepared, and the levels of estrogen and progesterone in cystic fluids were measured using the ELISA method. The ultrasound evaluations revealed a statistically significant difference in follicle diameter among the control group and the luteal and follicular cystic groups. A significant increase was also seen in the mean follicular wall diameter, luteal, control and follicular cystic groups. Moreover, a significant difference in progesterone concentration was also observed between the control and the luteal cystic groups (p<0.05). During imaging, the cystic wall diameters were 2.5 mm, and they must be considered as follicular cysts (the wall diameter of <3 mm criterion); surprisingly, they were classified as luteal cysts in the histopathological evaluation. In conclusion, according to the pathological examination, 3 mm of wall thickness is not a good criterion for the diagnosis of ovarian cyst type, because luteal cysts may have a wall diameter of less than 3 mm.

Keywords

Main Subjects

References

  1. Mimoune N, Baazizi R, Azzouz MY, Benaissa MH, Kaidi R. Basic and new concepts of ovarian cyst pathogenesis in cattle. Veterinaria. 2019; 68:73-80.
  2. Motta RG, Martins L, Medeiros GS, Chiogna Junior V. Prevalence of ovarian cysts in production high cows under containment system and diet total. Rev Cienc Agrov. 2019; 18(4):519-25.
  3. ElbehiryMA, Mahboob M, Marey M, Hattab S, Ghallab R, Dohreig R. Efficacy of three hormonal treatments for cystic ovarian disease and subsequent reproductive performance in dairy cows. Damanhour J Vet Sci. 2022; 8(1):6-10. https://doi.org/10.21608/djvs.2021.99394.1053
  4. Roman L, Broshkov M, Popova I, Hierdieva A, Sidashova S, Bogach N, et al. Influence of ovarian follicular cysts on reproductive performance in the cattle of new Ukrainian red dairy breed. Ukr J Ecol. 2020; 10(2):426-34.
  5. Mimoune N, Benaissa MH, Baazizi R, Radhwane S, Yassine AM, Ayed B, et al. Histological and immune-histochemical evaluation of ovarian cysts in cattle. Rumin Sci. 2020; 9(1):1-6.
  6. Gareis N, Rodríguez F, Moreyra MC, Stassi A, Angeli E, Etchevers L, et al. Contribution of key elements of nutritional metabolism to the development of cystic ovarian disease in dairy cattle. Theriogenology. 2022;197:209-23. https://doi.org/10.1016/j.theriogenology.2022.12.003
  7. Stassi A, Díaz P, Gasser F, Velázquez M, Gareis N, Salvetti N, et al. A review on inflammation and angiogenesis as key mechanisms involved in the pathogenesis of bovine cystic ovarian disease. Theriogenology. 2020; 186:70-85. https://doi.org/10.1016/j.theriogenology.2022.04.002
  8. Jeyakumar S, De AK, Kundu A, Roy K, Sunder J, Kundu M, et al. Sonographic characteristics of goat testis on water bath based ultrasonography. Livest Sci. 2013; 152(1):79-87. https://doi.org/10.1016/j.livsci.2012.11.010
  9. Dolecheck K, García-Guerra A, Moraes L. Quantifying the effects of mastitis on the reproductive performance of dairy cows: a meta-analysis. J Dairy Sci. 2019; 102(9):8454-77. https://doi.org/10.3168/jds.2018-15127
  10. Chauhan J, Hadiya K, Dhami A, Sarvaiya N. Ovarian dynamics, plasma endocrine profile and fertility response following synchronization protocols in crossbred cows with cystic ovaries. Indian J Anim Res. 2021; 55(2):127-33. https://doi.org/10.18805/ijar.B-3944
  11. Diskin M, Mackey D, Roche J, Sreenan J. Effects of nutrition and metabolic status on circulating hormones and ovarian follicle development in cattle. Anim Reprod Sci. 2003; 78(3-4):345-70. https://doi.org/10.1016/S0378-4320(03)00099-X
  12. Warma A, Descarreaux M, Chorfi Y, Dupras R, Rémillard R, Ndiaye K. Interleukins’ expression profile changes in granulosa cells of preovulatory follicles during the postpartum period in dairy cows. Cytokine. 2020; 2(1):100022. https://doi.org/10.1016/j.cytox.2020.100022
  13. Khalil AY. Comparative efficacy of two synchronization of ovulation regimens in the treatment of cystic ovarian disease in dairy cows. Alex J Vet Sci. 2019; 62(1):61-71. https://doi.org/10.5455/ajvs.47375
  14. Youngquist RS, Threlfall WR. Large animal theriogenology. St. Louis (MO): Saunders; 2017.
  15. Guadagnin A, Fehlberg L, Thomas B, Sugimoto Y, Shinzato I, Cardoso F. Effect of feeding rumen-protected lysine through the transition period on postpartum uterine health of dairy cows. J Dairy Sci. 2022; 105(9):7805-19. https://doi.org/10.3168/jds.2022-21934
  16. Turner Z, Lima F, Conley A, McNabb B, Rowe J, Garzon A, et al. Cystic ovarian disease in dairy cattle: Diagnostic accuracy when using B-mode and color Doppler ultrasound. J Dairy Sci. 2023; 106(5):3411-20. https://doi.org/10.3168/jds.2022-22498
  17. Mimoune N, Azzouz M Y, Khelef D, Kaidi R. Ovarian cysts in cattle: a review. Vet Stanica. 2021; 52(5):587-603. https://doi.org/10.46419/vs.52.5.10
  18. Dhara S, SharmaM. Cystic ovarian disease in dairy cow. Theriogenology Insight. 2019; 9(1):27-34.
  19. Ali A, Derar DR, Zeitoun M M,Al-Sobayil F. The interrelationship between the occurrence of oversized follicles and the peripheral and intra-follicular concentrations of E2, P4, FSH, and LH in female dromedary camels. J Camel Health. 2019; 1:11-19.
  20. Gupta C, Satheshkumar S, Murugan M, RamprabhuR. Management of Follicular Cyst by multiple approaches in crossbred cows. J Livest Res. 2020; 10:164-8. http://dx.doi.org/10.5455/ijlr.20200507083031
  21. Rudowska M, Baranski W, Zdunczyk S, Tobolski D, Janowski T. Effect of progesterone-releasing intravaginal device on reproductive performance of cows with cystic ovarian follicles after puerperium. Pol J Vet Sci. 2019; 22(3):489-94.
  22. Abdalla H, de Mestre AM, SalemSE. Efficacy of ovulation synchronization with timed artificial insemination in treatment of follicular cysts in dairy cows. Theriogenology. 2020;154:171-80. https://doi.org/10.1016/j.theriogenology.2020.05.029
  23. Yoshida R, Kitahara G, Osawa T. Intrauterine infusion of povidone-iodine: Its effect on the endometrium and subsequent fertility in postpartum dairy cows. J Vet Med Sci. 2020; 82(7):926-34. https://doi.org/10.1292/jvms.20-0165
  24. Jeengar K, Chaudhary V, Kumar A, Raiya S, Gaur M, PurohitG. Ovarian cysts in dairy cows: old and new concepts for definition, diagnosis and therapy. Anim Reprod. 2018; 11(2):63-73.
  25. Channo A, Kaka A, Kalwar Q, Jamali I, Jelani G, Bakhsh M, Dahri G N,Goil J P. An overview of bovine cystic ovarian disease. Pak J Zool. 2022; 54(5):24-37. https://doi.org/10.17582/journal.pjz/20210905140956
  26. Pirestani A, Jamali F. Relationship between follicular and luteal ovarian cysts with blood sugar, insulin and IGF-1 in multiparous cows. Vet Res Biol Prod. 2018; 31(4):9-17.
  27. Samadi F, Zanganeh K, Hasani S, Jafari AY. Comparison of metabolites levels and hormones of estrogen, progesterone, insulinand insulin-like growth factor-1 in blood serum and follicular fluid of mature and cystic follicles in dairy cows. Anim Sci Res. 2013; 23(3):41-50.
  28. Barański W, Gajęcka M, Zielonka Ł, Mróz M, Onyszek E, Przybyłowicz KE, et al. Occurrence of zearalenone and its metabolites in the blood of high-yielding dairy cows at selected collection sites in various disease states. Toxins. 2021; 13(7):446. https://doi.org/10.3390/toxins13070446
  29. Gareis N C, Stassi AF, Huber E, Rodríguez FM, Moreyra MLC, Salvetti NR, et al. Alterations in the insulin signaling pathway in bovine ovaries with experimentally induced follicular persistence. Theriogenology. 2020; 158:158-67. https://doi.org/10.1016/j.theriogenology.2020.09.016
  30. Engiz O, Berberoglu M, Siklar Z, Bilir P, Ocal G. Treatment of autonomous ovarian follicular cyst with long-term anastrozole therapy. Indian J Pediatr. 2009; 76:950-51. https://doi.org/10.1007/s12098-009-0190-2
  31. Stassi AF, Gareis NC, Marelli BE, Matiller V, Leiva J, Rey F, et al. Follicular structures of cows with cystic ovarian disease present altered expression of cytokines. Zygote. 2019; 27(5):285-98. https://doi.org/10.1017/S0967199419000285
  32. Rodríguez FM, NotaroUS, Huber E, Recce S, Ortega HH, Signorini ML, et al. Association between heat stress during intrauterine development and the expression and regulation of ovarian steroid hormone receptors in adult Holstein cows. Reprod Fertil Dev. 2022; 34(16):1003-22. https://doi.org/10.1071/rd22067
  33. Song Y, Cheng J, Yu H, Wang Z, Bai Y, Xia C, et al. Early warning for ovarian diseases based on plasma non-esterified fatty acid and calcium concentrations in dairy cows. Front Vet Sci. 2021; 8:792498. https://doi.org/10.3389/fvets.2021.792498
Veterinary and Comparative Biomedical Research
Volume 3, Issue 1
March 2026
Pages 29-36

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