Investigating the analgesic effects of combined diphenhydramine and dipyrone in mice

Document Type : Original Article

Authors

1 Graduated from the Faculty of Veterinary Medicine, Babol branch, Islamic Azad University, Babol, Iran

2 Department of Basic Sciences, Faculty of Veterinary Medicine, Babol branch, Islamic Azad University, Babol, Iran

3 Graduated from the Faculty of Sciences, Neyshabour branch, Islamic Azad University, Neyshabour, Iran

Abstract

Pain relief is a crucial aspect of modern surgery. The analgesic used should possess strong analgesic properties, a rapid onset of action, and minimal side effects to effectively reduce pain during and after surgical procedures. Therefore, the aim of the present study was to evaluate the analgesic effects of diphenhydramine and dipyrone (metamizole) in mice. For this study, 30 mice were randomly divided into five groups: the control group received normal saline; the standard group received 10 mg/kg morphine; treatment group 1 received 2.5 mg/kg diphenhydramine; treatment group 2 received 2.5 mg/kg dipyrone; and treatment group 3 received 2.5 mg/kg diphenhydramine combined with 2.5 mg/kg dipyrone. After injection, pain was assessed using the hot plate test and the formalin test. The results from both tests indicated that the combined use of dipyrone and diphenhydramine produced significant analgesic effects. Additionally, neither dipyrone (metamizole) nor diphenhydramine exhibited any side effects. Therefore, the use of dipyrone (metamizole) and diphenhydramine is recommended for safer surgical procedures.

Keywords

References

  1. Lin HC, Riddell MG. (2003). Preliminary study of the effects of xylazine or detomidine with or without butorphanol for standing sedation in dairy cattle, Vet Ther; 4(3):285-91.
  2. Borra RC, de Mesquita Barros F, de Andrade Lotufo M, Villanova FE, Andrade PM. (2009). Toll-like receptor activity in recurrent aphthous ulceration. J Oral Pathol Med, 38(3):289-98.
  3. Guimarães AL, Correia-Silva Jde F, Sa AR, Victória JM, Diniz MG, Costa Fde O, et al. (2007). Investigation of functional gene polymorphisms IL-1beta, IL-6, IL-10 and TNF-alpha in individuals with recurrent aphthous stomatitis, Arch Oral Biol; 52(3):268-72.
  4. Rezende, R.M. Franca, D.S. Menezes, G.B. and Bakhie, Y.S. (2007). Different mechanisms underlie the analgesic actions of paracetamol and dipyrone in a rat model, British Veterinary Journal of Pharmacology, 153(4): 760-768.
  5. Sánchez, S.; De La Lastra, CA. Ortiz, P.; Motilva, V. & Martín, MJ. (2002). Gastrointestinal tolerability of metamizol, acetaminophen, and diclofenac in subchronic treatment in rats, Digestive Diseases and Sciences, 47(12), 2791- 2798.
  6. Sokolov, A.Y. and Sivachenko, I. and Panteleev, S.S. (2014). Effect of intravenous metamizol on ongoing and evoked activity of dura–sensitive thalamic neurons in rats, Journal of Veterinary Anesthesia and Analgesia, 4(1): 31-32.
  7. Zhang, Y. and Wang, X. (2014). Dipyrone inhibits neuron cell death. Journal of Pet Medicine, 69(4): 942-956.
  8. Jedziniak, P., Pietruk, K. and Olejnik, M. (2013). Rapid method for the determination of metamizol residues in bovine muscle by LC – MS / MS, Journal of Veterinary Anesthesia and Analgesia, 2(6): 82-84.
  9. Naga, R., Anjaneyulu, N. and Canesh, M.N. (2012). Evaluated the analgesic activity of metamizole and paracetamol, Journal of Advanced Pharmacology Researches, 3(1): 1-4.
  10. Flor, B., Yazbek, V.B. and Keila, K. (2013). Tramadol plus metamizol combined or not with NSAIDS is clinically effective for moderate to severe chronic pain, Journal of Veterinary Anesthesia, 11(10): 21-23.
  11. Patel, C.V. and Koppikar, M.G. (2014). Management of pain after abdominal surgery.Journal of Clinical Sciences, 10(2): 351-353.
  12. Japr, A. (2012). Monitoring of anesthesia depth in birds and reported that the corneal reflex in birds, Journal of Poultry Science, 27(5): 5-9.
  13. Farzin D, Saadati R. (2004). The effect of diphenhydramine on the neuromuscular transmission of the chick biventer cervicis muscle preparation, J Mazandaran Univ Med Sci; 14 (44):1-13.
  14. Liu W., Yang X., Li C., Mo A. Adverse drug reactions to local anesthetics: a systematic review, Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology. 2013; 115(3):319–327.

15. Fujita T, Ohue M, Fujii Y, Jotoku T, Miyauchi A, Takagi Y, Tsuchiya M, Endo Y. Prompt analgesic effect of antihistaminic diphenhydramine ointment on bone-joint-muscle pain as assessed by skin impedance, Pharmacology. 2013; 92(3-4):158-66. Doi: 10.1159/000354151. Epub 2013 Sep 5. PMID: 24008505.

Veterinary and Comparative Biomedical Research
Volume 1, Issue 2
December 2024
Pages 66-71

Files

Share

How to cite

Statistics