Antibacterial activity of ethanolic and aqueous extract of Ajwain (Trachyspermum ammi) and Mountain tea (Stachys lavandulifolia) against Lactococcus garvieae strains

Document Type : Original Article

Authors

1 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

2 Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran

Abstract

The widespread use of antibiotics in agriculture, aquaculture, and medicine has led to the environmental dispersion of these compounds and the rise of drug-resistant bacterial pathogens. To prevent antibiotic resistance, employing herbal extracts and essential oils against pathogenic bacteria is a promising strategy. This study evaluated the antimicrobial efficacy of plant extracts from Trachyspermum ammi (T. ammi) and Stachys lavandulifolia (S. lavandulifolia) against Lactococcus garvieae (L. garvieae) using the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and disc diffusion test. Results showd that the ethanolic extract of T. ammi showed inhibition zones ranging from 22.06 mm to 0 mm, with an MBC of 18.75 mg/mL, while the aqueous extract ranged from 18.2 mm to 0 mm, with an MBC of 75 mg/mL. For comparison, the inhibition zones for streptomycin, erythromycin, and gentamicin were 21.75, 20.7, and 18 mm for the ethanolic extract and 21.32, 19.9, and 18.5 mm for the aqueous extract, respectively. In the case of S. lavandulifolia, the ethanolic extract exhibited inhibition zones ranging from 17.2 mm to 0 mm, with an MBC of 75 mg/mL, while the aqueous extract ranged from 16.6 mm to 0 mm, also with an MBC of 75 mg/mL. The inhibition zones for streptomycin, erythromycin, and gentamicin were 22.3, 18.9, and 18.1 mm for the ethanolic extract and 21.8, 20.2, and 17.9 mm for the aqueous extract, respectively. In conclusion, both T. ammi and S. lavandulifolia possess significant antimicrobial activity against L. garvieae, particularly at higher concentrations. Ethanolic extracts generally demonstrated greater efficacy compared to aqueous extracts, suggesting ethanol as a more effective solvent for extracting antimicrobial compounds. These findings support the potential use of these plant extracts as alternative treatments in aquaculture to combat bacterial infections and reduce reliance on traditional antibiotics, thereby mitigating the risk of antibiotic resistance

Keywords

References

  1. Vendrell D, Balcázar JL, Ruiz-Zarzuela I, De Blas I, Gironés O, Múzquiz JL. Lactococcus garvieae in fish: a review. Comp Immunol Microbiol Infect Dis. 2006;29(4):177–98.
  2. Tariq EF, Irshad Y, Khalil HB, Khakwani AS, Khan UA. Urinary tract infection caused by the novel pathogen, Lactococcus garvieae: a case report. Cureus. 2020;12(7).
  3. Pastorino P, Vela Alonso AI, Colussi S, Cavazza G, Menconi V, Mugetti D, et al. A summer mortality outbreak of Lactococcosis by Lactococcus garvieae in a raceway system affecting farmed rainbow trout (Oncorhynchus mykiss) and brook trout (Salvelinus fontinalis). Animals. 2019;9(12):1043.
  4. Soltani M, Baldisserotto B, Hosseini Shekarabi SP, Shafiei S, Bashiri M. Lactococcosis a re-emerging disease in aquaculture: Disease significant and phytotherapy. Vet Sci. 2021;8(9):181.
  5. Laws M, Shaaban A, Rahman KM. Antibiotic resistance breakers: current approaches and future directions. FEMS Microbiol Rev. 2019;43(5):490–516.
  6. Tiku AR. Antimicrobial compounds and their role in plant defense. Mol Asp plant-pathogen Interact. 2018;283–307.
  7. Chahal KK, Dhaiwal K, Kumar A, Kataria D, Singla N. Chemical composition of Trachyspermum ammi L. and its biological properties: A review. J Pharmacogn Phytochem. 2017;6(3):131–40.
  8. Bahadori MB, Zengin G, Dinparast L, Eskandani M. The health benefits of three Hedgenettle herbal teas (Stachys byzantina, Stachys inflata, and Stachys lavandulifolia)-profiling phenolic and antioxidant activities. Eur J Integr Med. 2020;36:101134.
  9. Cockerill FR. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard. (No Title). 2012;
  10. Khan NT, Jameel N. Screening of Trachyspermum ammi antibacterial activity. Biochem Anal Biochem. 2018;7(359):1009–2161.
  11. Jafari-Sales A, Rasi-Bonab F, Sayyahi J. The survey on antimicrobial effects of methanolic extract of Carum copticum L. on Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa in laboratory conditions. Paramed Sci Mil Heal. 2019;13(4):19–25.
  12. Javan AJ, Salimiraad S, Khorshidpour B. Combined effect of Trachyspermum ammi essential oil and propolis ethanolic extract on some foodborne pathogenic bacteria. In: Veterinary Research Forum. Faculty of Veterinary Medicine, Urmia University, Urmia, Iran; 2019. p. 235.
  13. Mood BS, Shafeghat M, Metanat M, Saeidi S, Sepehri N. The inhibitory effect of ajowan essential oil on bacterial growth. Int J Infect. 2014;1(2).
  14. Hosseinkhani F, Jabalameli F, Banar M, Abdellahi N, Taherikalani M, Leeuwen WB van, et al. Monoterpene isolated from the essential oil of Trachyspermum ammi is cytotoxic to multidrug-resistant Pseudomonas aeruginosa and Staphylococcus aureus strains. Rev Soc Bras Med Trop. 2016;49:172–6.
  15. Lee HJ, Park SN. Antioxidative effect and active component analysis of Quercus salicina Blume extracts. J Soc Cosmet Sci Korea. 2011;37(2):143–52.
  16. Mohseni Sisakht P, Nemayollahi A, Mohebbi A, Rahimi R. Study on the effect of aqueous and ethanolic extracts of Juglans regia plant on Lactococcus garvieae isolated from meat of rainbow trout (Onchorynchus mykiss). J Food Microbiol. 2019;6(3):82–91.
  17. Taheri M, Mahdavi S, Mehmannavaz Y. Antibacterial activity of different extracts from the aerial parts and roots of Stachys lavandulifolia Vahl harvested at two stages of growth. J Med Herbs,. 2014;5(1):7–12.
  18. Bashyal S, Guha A. Evaluation of Trachyspermum ammi seeds for antimicrobial activity and phytochemical analysis. Evaluation. 2018;11(5):148–222.
  19. Miller SI, Salama NR. The gram-negative bacterial periplasm: Size matters. PLoS Biol. 2018;16(1):e2004935.

20.        Veisi H, Karmakar B, Tamoradi T, Hemmati S, Hekmati M, Hamelian M. Biosynthesis of CuO nanoparticles using aqueous extract of herbal tea (Stachys Lavandulifolia) flowers and evaluation of its catalytic activity. Sci Rep. 2021;11(1):1–13.

Veterinary and Comparative Biomedical Research
Volume 1, Issue 2
December 2024
Pages 51-57

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