The effect of Ketotifen on reducing the inflammatory response in Newcastle disease in chickens

Document Type : Original Article

Authors

1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord branch, Islamic Azad University, Shahrekord, Iran

2 Graduated from Faculty of Veterinary Medicine, Shahrekord branch, Islamic Azad University, Shahrekord- Iran

Abstract

In this study, the effect of ketotifen on Newcastle Disease (ND) lesions was evaluated. A total of 150 one-day-old Ross broiler chickens were randomly divided into 5 groups with three replicates. The chickens received Newcastle Disease Vaccine (NDV) and ketotifen with different regimens. Group 1, as a negative control, received no vaccine and virulent NDV. Group 2 received acute NDV and no vaccine. The chickens in group 3 received acute NDV and ketotifen at the same time. Group 4 received acute NDV and ketotifen (before and after virus administration). Group 5 received acute NDV and two doses of Lasota ND vaccine at 10 and 20 days of age. 24 h after challenge, 3 chickens from each group were randomly slaughtered and the proventriculus was collected in 10% formalin for histopathological examination. In addition, the proventriculus was collected 72 h after virus inoculation. Blood samples were collected 24 hours before and 1 week after NDV challenge to determine antibody titers against NDV. Mortality was monitored for 7 days after virus inoculation. Results showed that the comparison of HI antibody titer against NDV in different groups one week after challenge showed that there is a significant difference between groups 1 with others. The comparison of mast cell population in 24 h before challenge showed that the highest and lowest population of mast cells was seen in groups 1 and 5 (respectively), that there is a significant difference between these groups. In one week after challenge, there is no significant difference between all groups for mast cell population. NDV vaccination can increase the protection against NDV challenge. In addition, the administration of ketotifen can reduce the inflammatory process of NDV infection, causing less lesions and less mortality in chickens. 

Keywords

References

  1. Abdoshah M, Pourbakhsh SA, Peighambari SM, Shojadoost B, Momayez R, Mojahedi Z. Pathogenicity indices of Newcastle disease viruses isolated from Iranian poultry flocks in Iran. J Vet Res. 2012; 67(2):159-64.
  2. Bayram H, Devalia JL, Khair OA, Abdelaziz MM, Sapsford RJ, Czarlewski W, Campbell AM, Bousquet J, Davies RJ. Effect of loratadine on nitrogen dioxide–induced changes in electrical resistance and release of inflammatory mediators from cultured human bronchial epithelial cells. J Allergy Clin Immunol. 1999; 104(1):93-9.
  3. Panda A, Huang Z, Elankumaran S, Rockemann DD, Samal SK. Role of fusion protein cleavage site in the virulence of Newcastle disease virus. Microb pathol. 2004 1; 36(1):1-0.
  4. Dzogbema KF, Talaki E, Batawui KB, Dao BB. Review on Newcastle disease in poultry. Int. J. of Biol. and Chem. Sci. 2021; 15(2):773-89.
  5. Abraham SN, John AL. Mast cell-orchestrated immunity to pathogens. Nat Rev Immunol. 2010; 10(6):440-52.
  6. Amoia CF, Nnadi PA, Ezema C, Couacy-Hymann E. Epidemiology of Newcastle disease in Africa with emphasis on Côte d’Ivoire: A review. Vet World. 2021; 14(7):1727.
  7. Galli SJ, Kalesnikoff J, Grimbaldeston MA, Piliponsky AM, Williams CM, Tsai M. Mast cells as “tunable” effector and immunoregulatory cells: recent advances. Annu Rev Immunol. 2005; 23:749-86.
  8. Muhammadiy NS. Pengaruh Pemberian Ekstrak Kayu Secang (Caesalpinia Sappan L.) Terhadap Kadar Kolesterol Total Dan Trigliserida Darah Tikus Diabetes Yang Diinduksi Aloksan (Doctoral dissertation, Fakultas Farmasi Universitas Jember).
  9. Håkanson R, Rönnberg AL, Sjölund K. Fluorometric determination of histamine with OPT: optimum reaction conditions and tests of identity. Anal Biochem. 1972 1; 47(2):356-70.
  10. Jang J, Hong SH, Kim IH. Validation of a real-time RT-PCR method to quantify Newcastle disease virus (NDV) titer and comparison with other quantifiable methods. J Microbiol Biotechnol. 2011; 21(1):100-8.
  11. Marshall JS, Jawdat DM. Mast cells in innate immunity. J Allergy Clin Immunol. 2004; 114(1):21-7.
  12. Wernersson S, Pejler G. Mast cell secretory granules: armed for battle. Nat Rev Immunol. 2014; 14(7):478-94.
  13. Costa-Hurtado M, Afonso CL, Miller PJ, Shepherd E, DeJesus E, Smith D, & Pantin-Jackwood MJ. Effect of infection with a mesogenic strain of Newcastle disease virus on infection with highly pathogenic avian influenza virus in chickens. Avian Dis., 2016; 60(1), 269-278.
  14. Sun Q, Wang D, She R, Li W, Liu S, Han D, & Ding Y. Increased mast cell density during the infection with velogenic Newcastle disease virus in chickens. Avian pathol. 2008; 37(6), 579-585.
  15. Wang D, Xiong J, She R, Liu L, Zhang Y, Luo D, Li W, Hu Y, Wang Y, Zhang Q, Sun Q. Mast cell mediated inflammatory response in chickens after infection with very virulent infectious bursal disease virus. Vet Immunol Immunopathol. 2008 ; 124(1-2):19-28.
  16. Wang D, Zhou X, She R, Xiong J, Sun Q, Peng K, Liu L, Liu Y. Impaired intestinal mucosal immunity in specific-pathogen-free chickens after infection with very virulent infectious bursal disease virus. Poultry Sci. 2009; 88(8):1623-8.
  17. Gurish MF, Austen KF. Developmental origin and functional specialization of mast cell subsets. Immunity. 2012; 37(1):25-33.

19. Hu Y, Jin Y, Han D, Zhang G, Cao S, Xie J, Xue J, Li Y, Meng D, Fan X, Sun LQ. Mast cell-induced lung injury in mice infected with H5N1 influenza virus. J Virol. 2012; 86(6):3347-56

Veterinary and Comparative Biomedical Research
Volume 1, Issue 2
December 2024
Pages 1-7

Files

Share

How to cite

Statistics