Detection of Staphylococcus and Escherichia coli in the ocular microbiota of Persian cats

Document Type : Original Article

Authors

1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shoushtar branch , Islamic Azad University, Shoushtar, Iran

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Science and Research branch, Islamic Azad University, Tehran, Iran

3 Department of clinical Sciences, Faculty of Veterinary Medicine, Science and Research branch, Islamic Azad University, Tehran, Iran

Abstract

This study aimed to characterize the microbial and fungal flora in the eyes of 100 Persian cats and investigate their potential role in ocular health and disease in this breed. The composition and prevalence of microorganisms were determined using laboratory techniques such as culture and fungal isolation. The results revealed a diverse range of bacterial and fungal species present in the conjunctiva of Persian cats. Gram-positive bacteria, including Staphylococcus spp., Streptococcus spp., and Corynebacterium spp., were commonly identified as commensals, suggesting a healthy ocular microbiome. However, certain bacterial species associated with ocular disease in other feline breeds were also found, such as Pseudomonas spp. The presence of pathogenic bacteria, including Staphylococcus aureus and Escherichia coli, highlights the potential role of specific species in the development and progression of ocular conditions in Persian cats. Fungal species, including Aspergillus spp., Candida spp., and Malassezia spp., were also detected, emphasizing the need to consider fungal etiologies in the diagnosis and management of ocular diseases in this breed. This knowledge will contribute to improved veterinary ophthalmology practices and aid in the diagnosis and treatment of ocular conditions in Persian cats and other feline breeds.

Keywords


  1. Baim AD, Movahedan A, Farooq AV, Skondra D. (2019) The microbiome and ophthalmic disease. Experimental Biology and Medicine, 244(6):419-29.
  2. Aftab G, Arfaee F, Asghari A, Zahraei Salehi T. (2023) The evaluation of normal ocular parameters in two breeds of hedgehogs. Veterinary Medicine and Science, 9(2):738-43.
  3. Aftab G, mehdi Rajaei S, Pot SA, Faghihi H. (2019) Seasonal effects on the corneoconjunctival microflora in a population of Persian cats in Iran. Topics in companion animal medicine, 34:30-2.
  4. Petrillo F, Petrillo A, Marrapodi M, Capristo C, Gicchino MF, Montaldo P, et al. (2022) Characterization and comparison of ocular surface microbiome in newborns. Microorganisms, 10(7):1390.
  5. Cooper S, McLellan G, Rycroft A. (2021) Conjunctival flora observed in 70 healthy domestic rabbits (Oryctolagus cuniculus). Veterinary Record, 149(8):232-5.
  6. Samuelson D, Brooks D. Small animal ophthalmology: Self-assessment color review: CRC Press; 2011.
  7. Meekins JM, Eshar D, Rankin AJ. (2015) Tear production, intraocular pressure, and conjunctival bacterial flora in a group of captive black‐tailed prairie dogs (C ynomys ludovicianus). Veterinary Ophthalmology., 18:132-6.
  8. Mirshahi A, Shafigh S, Azizzadeh M. (2014) Ultrasonographic biometry of the normal eye of the Persian cat. Australian veterinary journal, 92(7):246-9.
  9. Athanasiou L, Psemmas D, Papaioannou N. (2018) Conjunctival cytology assessment in dogs and cats. Sampling, diagnostic techniques and findings. Journal of the Hellenic Veterinary Medical Society, 69(1):701-10.
  10. Aslan O, Teberlk K, Yucel M, Gur N, Karakoc A. (2008) Effect of topical netilmicin on the reduction of bacterial flora on the human conjunctiva. European journal of ophthalmology, 18(4):512-6.
  11. Whitley RD. (2000) Canine and feline primary ocular bacterial infections. Veterinary Clinics of North America: Small Animal Practice, 30(5):1151-67.
  12. Gerding PA, Kakoma I. (1990) Microbiology of the canine and feline eye. Veterinary Clinics of North America: Small Animal Practice, 20(3):615-25.
  13. Elmenshawy YM, Ali KM, Samir A. (2021) Current Evidence of Coryneform Bacteria on The Ocular Surface of Immunocompromised Cats. Journal of Applied Veterinary Sciences, 6(3):86-93.
  14. Teweldemedhin M, Gebreyesus H, Atsbaha AH, Asgedom SW, Saravanan M. (2017) Bacterial profile of ocular infections: a systematic review. BMC ophthalmology, 17(1):1-9.
  15. Arteaga K, Aftab G, Rajaei SM, Faghihi H, Crasta M. (2021) Comparison of conjunctival microbiota of clinically normal Persian cats with and without nasolacrimal duct obstruction. Veterinary Ophthalmology, 24(5):455-9.
  16. Büttner JN, Schneider M, Csokai J, Müller E, Eule JC. (2019) Microbiota of the conjunctival sac of 120 healthy cats. Veterinary ophthalmology, 22(3):328-36.