Shahid Bahonar University of Kerman Veterinary and Comparative Biomedical Research 3060-7663 2 1 2025 06 01 The Effects of the Curcumin-Loaded Selenium Nanoparticles on Hepatic Stereo-Histology, Blood Glucose, and Lipid Profile in Rats 1 9 4565 10.22103/vcbr.2024.24246.1027 EN Jahangir Kaboutari Department of Basic Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran Rahmat Allah Fatahian Dehkordi Department of Basic Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran Mehdi Amirkhani Department of Basic Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran Pegah Khosraviyan Medicinal Plant Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran Journal Article 2024 10 04 The application of novel pharmaceutical formulations of natural compounds, including those incorporating nanoparticles, represents a promising avenue for therapeutic advancement. The objective of this study was to examine the impact of curcumin-loaded selenium nanoparticles on the hepatic histological structure, as well as on blood glucose and lipids in rats. A total of 75 adult male rats were randomly assigned to one control group and four treatment groups. The control group was administered no medication, while the four treatment groups received the following: 50 mg/kg of curcumin (Group 1), 3.5 mg/kg of selenium nanoparticles (Group 2), combination of 50 mg/kg of curcumin plus 3.5 mg/kg of curcumin-loaded selenium nanoparticles (Group 3), and 5% DMSO (Group 4). On days 3, 7, and 14, blood samples were collected for biochemical testing and liver tissue samples were obtained. The hepatic tissue structure of the control, curcumin, and solvent groups exhibited no significant differences on days 3, 7, and 14. It was observed that the selenium nanoparticles group exhibited notable alterations in tissue structure, which were markedly diminished in the curcumin-loaded selenium nanoparticles group. Notable stereological findings were observed in the selenium nanoparticles group in comparison to the control group, including an increase in the volume density of hepatocytes and sinusoids, accompanied by a reduction in the volume density of central veins. No significant alterations were observed in the triglyceride and cholesterol levels in the experimental groups. It can be concluded that the administration of curcumin-loaded selenium nanoparticles to rats demonstrated notable hepatoprotective effects against selenium nanoparticles.

https://vcbr.uk.ac.ir/article_4565_8993e213cd3496ce0f55027775570a03.pdf

Shahid Bahonar University of Kerman Veterinary and Comparative Biomedical Research 3060-7663 2 1 2025 06 01 The First Molecular Screening of Equine Leptospiral Infection in Iran 10 15 4579 10.22103/vcbr.2024.24294.1028 EN Marjan Khorasaninejad Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran Elham Mohammadi Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran Ehsanollah Sakhaee Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran 0000-0003-3496-5532 Journal Article 2024 10 03 Leptospira is one of the spiral-shaped Gram-negative bacteria, which includes pathogenic (Leptospira interrogans) and non-pathogenic (Leptospira biflexa) species. This bacterium is zoonotic and is scattered all over the world. It infects a wide range of hosts, including humans, domestic animals and, rodents. Investigation and identification of Leptospira in horses is often neglected or less addressed. However, this infection in horses can cause significant economic losses, such as the loss of expensive foals of rare breeds. Leptospirosis in horses is often subclinical and shows fewer clinical symptoms, especially in adults, but in acute cases, it is associated with jaundice, hemoglobinuria, renal dysfunction, lethargy, periodic ophthalmia and, abortion in mares. Therefore, it is necessary to investigate Leptospiral infection in horses. For this purpose, 100 blood samples with anticoagulants were taken from the jugular veins of clinically healthy horses in Kerman province, Iran. Then, using the DNA extraction kit, according to the manufacturer's instructions, the DNA of the samples was extracted. In the following, a PCR technique using specific primers of the 16SrRNA gene of Leptospira interrogans was used to trace Leptospira bacteria. No positive samples were found. Based on these results, it can be concluded that Leptospira bacteria likely do not exist in the horses of Kerman city, Iran.

https://vcbr.uk.ac.ir/article_4579_f8003daaae0a458acd8e9055157b37d3.pdf

Shahid Bahonar University of Kerman Veterinary and Comparative Biomedical Research 3060-7663 2 1 2025 06 01 Toxoplasma gondii in Goats: A Case Report 16 18 4591 10.22103/vcbr.2024.24360.1029 EN Behrooz Mihandoost Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran Mohammad Saleh Fathi Saghezchi Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran Yazdad Yazdandoost Hamedani Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran Journal Article 2024 10 12 Abstract Toxoplasma gondii is a zoonotic disease agent with the cats as its definitive hosts; it can infect various animals, including sheep, goats, cattle, and birds. Toxoplasma gondii can cause early embryonic death, resorption, fetal death, mummification, abortion, stillbirth, and neonatal death depending on the stage of gestation. Implementing preventive measures play a significant role in controlling this disease. Six goats from an 80-goat industrial farm were referred to the veterinary clinic at Shahid Chamran University of Ahvaz due to incidents of abortion. In this report, after conducting detailed examinations and tests, the presence of Toxoplasma gondii was confirmed as the sole causative agent of abortion. Under the microscope, cysts of Toxoplasma gondii were clearly observed in brain samples from the aborted fetuses. Since this disease is zoonotic, to prevent infection, humans, especially pregnant women, are advised to avoid contact with suspicious animals and refrain from consuming unpasteurized milk and dairy products (especially goat milk), raw water, and contaminated water with cysts or parasites, as well as raw seafood.

https://vcbr.uk.ac.ir/article_4591_b0531fa20a2b25dbd81c3feb9442b699.pdf

Shahid Bahonar University of Kerman Veterinary and Comparative Biomedical Research 3060-7663 2 1 2025 06 01 Renal Amyloidosis in Cattle: An Abattoir-Based Pathological Study in Urmia, Iran 19 28 4653 10.22103/vcbr.2025.24452.1030 EN Hassan Ghorbanalizadeh Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran Amir Amniattalab Department of Pathology, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran Moslem Neyriz Naghadehi Department of Food Hygiene, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran Journal Article 2024 10 28 Serum-associated amyloidosis is the most common form of amyloidosis in domestic animals and can lead to death. In this study, 1294 cattle (with a total of 2588 kidneys inspected) were inspected at the Sahand Abattoir, Urmia, Iran, over one year. Of these, 2462 kidneys were healthy (95.1%) while 126 kidneys had lesions (4.9%). There was no significant correlation between the frequency of gross lesions in the kidney and the sex or breed of the cattle (p>0.05). Additionally, the frequency of macroscopic changes in all cattle aged 4.5 years and older was significantly higher than that of younger ones and calves (p<0.05). Microscopic examination using hematoxylin and eosin, periodic acid schiff, and congo red tissue staining methods revealed the relative frequency of kidney lesions as follows: hemorrhage (27%), hydropic degeneration (16.7%), congestion (15.9%), interstitial nephritis (8.7%), glomerulonephritis (7.1%), acute tubular necrosis (6.3%), hydronephrosis (4.8%), cysts (4%), fibrosis (3.2%), amyloidosis (1.6%), pyelitis (1.6%), and renal lipidosis (0.8%). In two kidneys with amyloid deposits, one belonged to a bull with localized pulmonary tuberculosis, while the other belonged to a different bull with no other lesions detected in any organs. The most involved structures were glomeruli (98%), and only 2% of cortical tubules had amyloid deposits. Although renal amyloidosis is rare in slaughtered cattle in Urmia city, its heat resistance and potential transmission through food, especially to individuals with chronic inflammatory diseases, require careful inspection in slaughterhouses and condemnation of suspicious carcasses.

https://vcbr.uk.ac.ir/article_4653_ec2b19e097b8f9211c5127bdbc0dc6ba.pdf

Shahid Bahonar University of Kerman Veterinary and Comparative Biomedical Research 3060-7663 2 1 2025 06 01 Evaluation of the Combined Analgesic Effect of Diphenhydramine and Ketoprofen Compared to Morphine in Mice 29 34 4703 10.22103/vcbr.2025.24547.1032 EN Firouz Faedmaleki Department of Pharmacology, Faculty of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, Iran 0000-0002-8532-536X Mohsen Alami Rostami Department of Clinical sciences, Faculty of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, Iran Mostafa Kalantari Department of Clinical sciences, Faculty of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, Iran Arya Soneishargh Faculty of Sciences, Neyshabour Branch, Islamic Azad University, Razavi Khorasan, Iran Journal Article 2024 12 04 Morphine is a significant and effective pain relief medication that has been of interest for a long time. However, the rapid onset of tolerance and dependence on morphine and other opioids remains a critical limiting factor in their use. Numerous studies have investigated the mechanisms involved in opioid dependence, leading researchers to focus on alternative medications with fewer side effects. The aim of the present study was to investigate the combined analgesic effects of diphenhydramine and ketoprofen compared to morphine in mice. Thirty mice were randomly divided into five groups of six and analgesia was induced through intraperitoneal injection as follow, control group (normal saline), standard group receiving 10 mg/kg morphine, treatment group 1 receiving 2.5 mg/kg diphenhydramine, treatment group 2 receiving 2.5 mg/kg ketoprofen, and treatment group 3 receiving 2.5 mg/kg diphenhydramine plus 2.5 mg/kg ketoprofen. Following injection, pain assessment was conducted using the hot plate test and formalin tests. The results indicated that the combined use of ketoprofen and diphenhydramine exhibited significant analgesic effects compared to the control group with fewer side effects, suggesting they could serve as alternatives to morphine. However, further studies are needed to evaluate the side effects of ketoprofen and diphenhydramine.

https://vcbr.uk.ac.ir/article_4703_9460d9e00ed205bfb4a74e53d3bbd461.pdf

Shahid Bahonar University of Kerman Veterinary and Comparative Biomedical Research 3060-7663 2 1 2025 06 01 In Vitro Evaluation of Methanolic Extracts of Urtica dioica and Melissa officinalis as Scolicidal Agents Against Hydatid Cyst Protoscoleces 35 42 4707 10.22103/vcbr.2025.24622.1036 EN Fereshteh Ranjbar Shourabadi Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran Mohammad Hossein Radfar Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran Mohammad Mirzaei Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran 0000-0003-1829-1478 Soheila Fotoohi Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran Mehran Mesgari Abbasi Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Mohammad Ali Eslami Meymand Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran Journal Article 2024 12 03 Cystic Echinococcosis is a zoonotic infection that causes complications such as cyst rupture and secondary infections, requiring effective scolicidal agents. Due to adverse reactions and toxicity of current treatments, new plant-derived SA are needed. This study evaluates the scolicidal effects of methanolic extracts of Urtica dioica and Melissa officinalis in vitro. Leaves were dried, ground, soaked in methanol, filtered, and concentrated. protoscoleces of Echinococcus granulosus were isolated from an infected sheep's liver and assessed for viability using eosin staining. The efficacy of plant extracts was tested at concentrations of 50, 100, and 200 mg/ml for 15, 30, 45, 60, and 120 minutes. Positive controls were 70% ethanol and 20% sodium chloride; 0.9% normal saline served as the negative control. Results showed that 70% ethanol and 20% sodium chloride had a lethal effect of 86-100%. Urtica extract had a 22-72% lethal effect at 200 mg/ml, 8-40% at 100 mg/ml, and 7-32% at 50 mg/ml. Melissa extract had a 37-95% lethal effect at 200 mg/ml, 16-52% at 100 mg/ml, and 12-37% at 50 mg/ml. The results showed that the protoscolexicidal activity of methanol extracts increased with time. The methanol extract of lemon balm at a concentration of 200 mg/ml killed 95% of protoscoleces in 120 minutes, while the nettle extract at the same concentration killed 72% of protoscoleces in the same period. Therefore, the methanol extract of lemon balm can be utilized as a potent protoscolexicidal agent.

https://vcbr.uk.ac.ir/article_4707_c28bca2b078c3a6e9eaa22658bd3e0ff.pdf

Shahid Bahonar University of Kerman Veterinary and Comparative Biomedical Research 3060-7663 2 1 2025 06 01 Prevalence of Oestrus ovis Larval Infestation in the Slaughtered Sheep Population of Sardasht County, West Azerbaijan, Iran 43 50 4709 10.22103/vcbr.2025.24509.1031 EN Khezr Hassanzadeh Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran Omid Mohammadpour Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran 0009-0000-5103-3054 Sohrab Rasouli Department of Pathobiology, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran Journal Article 2024 12 12 Oestrus ovis, commonly known as the nasal botfly, has a global distribution and causes myiasis in sheep and goats. The present study aimed to evaluate the prevalence of Oestrus ovis larval infestation in the slaughtered sheep population of Sardasht County, West Azerbaijan, Iran. This cross-sectional study was conducted over a 12-month period at the local slaughterhouse. Each month, 34 sheep were examined, and data regarding gender, age, breed, and sampling time were analyzed. Among the 408 sheep examined, 138 cases (33.8%) were found positive for infestation. The highest prevalence was observed in sheep older than three years (16.7%), while the lowest was recorded in sheep younger than one year (2.4%), indicating a significant correlation between infestation rate and age (P < 0.05). The highest prevalence was observed in summer (11.0%) and the lowest in winter (5.9%). The month-wise analysis showed the peak infestation in August (3.9%) and the lowest in February (1.7%), with a statistically significant seasonal pattern (P < 0.05). However, no significant association was found between infestation rate and sheep gender or breed (P > 0.05). This study highlights Oestrus ovis as a prevalent parasite in Sardasht County, capable of causing substantial economic losses in sheep farming. Additionally, due to its zoonotic potential, the parasite poses a public health concern.

https://vcbr.uk.ac.ir/article_4709_f3b5ca106155d4508e7f37b9ede0b1f2.pdf

Shahid Bahonar University of Kerman Veterinary and Comparative Biomedical Research 3060-7663 2 1 2025 06 01 The Effects of Epigallocatechin-3-Gallate Extract Encapsulated by Bio-Polymeric Alginate-Chitosan Nanoparticles on Testicular Histopathology and Histomorphometry of Mice Following LPS-Induced Acute Endotoxemia 51 59 4726 10.22103/vcbr.2025.24696.1041 EN Ahad Soltani Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran Homayoon Babaei Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran Reza Kheirandish Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran Masoud Imani Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran Pourya Mohammadi Department of Chemistry, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran Journal Article 2025 01 02 Endotoxemia is a significant cause of mortality and abnormalities in animals, particularly neonates, within the first 24 hours of life. It is triggered by the release of lipopolysaccharide from the cell wall of gram-negative bacteria into the bloodstream. This study aimed to investigate the impact of epigallocatechin gallate (EGCG) in its simple form and as nanoparticles on the histopathological changes in the testes of NMRI mice induced by endotoxemia. Sixteen male mice were divided into four experimental groups. The control group received no treatment, while the other three groups were induced with acute endotoxemia by intraperitoneal injection of 6750 µg/kg of lipopolysaccharide. Group 3 received EGCG treatment (25 mg/kg) and group 4 received nano-EGCG treatment (25 mg/kg) intra-peritoneally once daily for 28 days. Evaluation of testicular tissue parameters, including spermatogenesis percentage, Johnsen's score, and meiotic index, showed improvement in the EGCG-treated groups, with no significant difference between the simple and nano forms. In conclusion, EGCG administration alleviates the adverse effects of acute endotoxemia on testicular tissue, with no significant difference observed between encapsulated and unencapsulated EGCG.

https://vcbr.uk.ac.ir/article_4726_d0d0bddfbc0942313d3bfd752f906169.pdf

Shahid Bahonar University of Kerman Veterinary and Comparative Biomedical Research 3060-7663 2 1 2025 06 01 Renal Function Assessment in Domestic Shorthair Cats Using Scintigraphy 60 65 4758 10.22103/vcbr.2025.24751.1045 EN Darioush Vosough Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran Nadya Nadimi Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran Amir Ghazi Behzadi Department of Clinical Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran Mehrdad Hamzehi Department of Clinical Sciences, Faculty of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, Iran Mohammad Shahriari Ahmadi Department of Clinical Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran Mohammadmahdi Alinaghizadeh Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran 0009-0000-9746-9283 Negar Karimi Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran Samin Sadat Batheai Department of Clinical Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran Journal Article 2025 01 29 Scintigraphy, also known as a gamma scan, is a diagnostic imaging procedure utilized in the field of nuclear medicine. This procedure involves the use of radioisotopes attached to drugs, which serve as tracers. These tracers facilitate the creation of images that reveal the anatomical composition of the patient's internal organs and tissues. Scintigraphy provides distinctive insights into renal function, thereby complementing anatomical details obtained from magnetic resonance imaging and computed tomography scans. Its clinical applications include the assessment of anomalies, infections, and the evaluation of kidney function. The objective of this study was to evaluate the effectiveness of scintigraphy technique in assessing kidney function in cats. To obtain scintigraphy information, six adult healthy male cats were selected. The cats were anesthetized, and a dose of 4 millicuries of the radiopharmaceutical 99mTc-DTPA was injected into the saphenous vein of the animal simultaneously with the start of the scan. The emitted radiation was recorded by the camera for 30 minutes. The results obtained revealed that the total glomerular filtration rate was 125.66 ± 5.03 mL/min, with exclusive glomerular filtration rates for the left and right kidneys being 51.27 ± 2.29 and 48.78 ± 2.75 mL/min, respectively. The peak activity times were recorded as 4.4 ± 0.43 and 4.6 ± 0.12 minutes for the left and right kidneys, respectively. The results obtained from this study are within the normal range, which show the reliability of scintigraphy as a non-invasive, rapid, and accurate tool for evaluating renal indices in veterinary medicine.

https://vcbr.uk.ac.ir/article_4758_f4dc07d14bd9ed682a676eb8841c2247.pdf

Shahid Bahonar University of Kerman Veterinary and Comparative Biomedical Research 3060-7663 2 1 2025 06 01 The Impacts of Folic Acid on Hematological Parameters in German Shepherd Dogs with Parvovirus-Induced Anemia 66 71 4786 10.22103/vcbr.2025.24710.1042 EN Erfan Fadaie Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Baft Branch, Baft, Iran Hosein Iravani Department of Basic Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Baft Branch, Baft, Iran Mohammad Mazrouei Sebdani Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Baft Branch, Baft, Iran Journal Article 2025 01 21 Canine parvovirus often causes anemia in dogs, presenting significant therapeutic challenges. This study evaluated the effectiveness of folic acid supplementation in improving hematological parameters in German Shepherd dogs with parvovirus-induced anemia. Forty German Shepherd dogs diagnosed with parvovirus-induced anemia were randomly assigned to two groups: the control group received standard treatment alone, and the experimental group was administered 5 mg of folic acid daily in addition to standard treatment for two weeks. Significant improvements were observed in hematocrit, hemoglobin, mean corpuscular volume, and mean corpuscular hemoglobin concentration in the experimental group (p<0.05). No significant differences were observed in mean corpuscular hemoglobin, white blood cell count, and thrombocyte count between the groups. Folic acid supplementation improved several hematological parameters in dogs with parvovirus-induced anemia, highlighting its potential as an adjunctive therapeutic approach.

https://vcbr.uk.ac.ir/article_4786_acd3a3dd486e003a1680c2d587e2b658.pdf

Shahid Bahonar University of Kerman Veterinary and Comparative Biomedical Research 3060-7663 2 1 2025 06 01 Sero-Epidemiological Survey of Brucellosis in Farm Animals in Khuzestan Province, Iran 72 79 4787 10.22103/vcbr.2025.24930.1049 EN Hamid Mahmoodipour Department of Nursing and Midwifery, Behbahan Branch, Islamic Azad University, Behbahan, Iran Seyedeh Neda Moghadasian Department of Clinical Sciences, Faculty of Veterinary Medicine, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran Seyedeh Ommolbanin Ghasemian Department of Veterinary, Behbahan Branch, Islamic Azad University, Behbahan, Iran 0000-0002-4805-3553 Ehsan Gharib Mombeni Department of Clinical Sciences, Faculty of Veterinary Medicine, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran Journal Article 2025 02 11 Brucellosis is one of the most important zoonotic diseases caused by different species of Brucella spp. This zoonotic disease has always been important from an economic and healthcare point of view, so many countries are trying to eradicate the disease from the farm animal population. The purpose of the present study was to investigate the sero-epidemiology of brucellosis in farm animals in Behbahan city, Khuzestan province, Iran. For this purpose, 330 serum samples were collected as the study population to track brucellosis prevalence. From March to June 2022, the samples were analyzed by Rose Bengal, Wright and 2-Mercaptoethanol tests. The results obtained in the present study indicated that 3.6% of the studied animals were infected with brucella bacteria. Examining the results of 2-Mercaptoethanol indicated that in general, 58.3% (7 samples) of the examined animals whose initial test results were positive had positive 2-Mercaptoethanol, which means chronic brucellosis was determined. There was no difference in the frequency of brucellosis between animal species (p>0.05). The contamination in female animals (2.6%) was significantly higher than that of male animals (p<0.05). The results of this study demonstrated that brucellosis was a prevalent disease among farm animals in Behbahan city. It is important to note that the widespread occurrence of brucellosis among farm animals could be distributed to the human population. This underscores the imperative for the implementation of an efficacious program to control, prevent and ultimately eradicate this infectious agent in this area.

https://vcbr.uk.ac.ir/article_4787_ae9a06f04be8fcb6a412542602e3bf0e.pdf

Shahid Bahonar University of Kerman Veterinary and Comparative Biomedical Research 3060-7663 2 1 2025 06 01 A Comparative Analysis of Double-Ovsynch and Ovsynch-Heat-Synch Protocols on Pregnancy Rates in Primiparous Dairy Cows 80 84 4788 10.22103/vcbr.2025.24640.1038 EN Reza Ghasemian Moghadam Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran Afshin Davasaz Tabrizi Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran Arian Narouiee Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran Omid Mohammadpour Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Urmia Branch, Urmia, Iran 0009-0000-5103-3054 Journal Article 2025 01 07 Today, ovulation synchronization methods are widely used to improve fertility in dairy cows. In this regard, this research was conducted in one of the dairy herds in East Azerbaijan province, Iran. The cows were randomly divided into two groups. Ovsynch-Heat-Synch Group: This group included 30 primiparous cows. One month after calving, provided the uterus was clean, the synchronization program commenced. On the first and ninth days, they received GnRH, and on the seventh day, PGF2α intramuscularly. One week later, they received GnRH again; seven days after that, they received PGF2α, and 24 hours later, 1 mg of estradiol benzoate intramuscularly. Cows that showed estrus after the estradiol injection were inseminated using the AM/PM method, while those that did not show estrus 48 hours after the estradiol injection were artificially inseminated (Timed Artificial Insemination, TAI). Double-Ovsynch Group: This group also included 30 primiparous cows. One month after calving, provided the uterus was clean, the synchronization program began. On the first and ninth days, they received GnRH analogue; on day seven, PGF2α was administered intramuscularly. One week later, they received GnRH analogue again; seven days later, PGF2α was given, and 48 hours later, they received GnRH analogue intramuscularly. Forced insemination was performed 16 to 24 hours after the last GnRH injection. Pregnancy diagnosis was conducted using ultrasound between days 30 to 35 and by rectal palpation between days 40 to 45 after artificial insemination. The results showed that the fertility rate was 43.3% (13 cows) in the Ovsynch-Heat-Synch group and 60% (18 cows) in the Double-Ovsynch group. In the statistical analysis, this difference was not significant (p>0.05). This study indicated that the new Ovsynch-Heat-Synch method does not have a significant superiority over the Double-Ovsynch method.

https://vcbr.uk.ac.ir/article_4788_bfd3f6ad520426ecb80ccbcd0fc5fb80.pdf

Shahid Bahonar University of Kerman Veterinary and Comparative Biomedical Research 3060-7663 2 1 2025 06 01 Prevalence of Staphylococcus aureus Contamination and Detection of Enterotoxin A Gene in Raw Barbecue Chicken Meat Sold in Babol City, Iran 85 90 4815 10.22103/vcbr.2025.24653.1039 EN Mahdi Sharifi Soltani Department of Veterinary Medicine, Faculty of Agriculture, Chalous Branch, Islamic Azad University, Chalous, Iran Zeinab Asgharzadeh Faculty of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, Iran Mostafa Kalantari Faculty of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, Iran Arya Soneishargh Faculty of Sciences, Neyshabour Branch, Islamic Azad University, Neyshabour, Iran Journal Article 2025 01 10 The application Staphylococcus aureus, producing enterotoxin, is recognized as the most common cause of staphylococcal food poisoning, leading to gastroenteritis, diarrhea, and vomiting. The presence of enterotoxin-producing genes in this bacterium can be the main reason for these symptoms. The aim of this study was to determine the prevalence of Staphylococcus aureus contamination and detection of enterotoxin A gene in raw barbecue chicken meat sold in Babol city, Iran. After collecting 60 samples of raw barbecue chicken meat, the samples were examined for the presence of Staphylococcus aureus using standard culture techniques. Following DNA extraction, PCR tests were conducted using specific primers to identify the sea gene. According to the results obtained, Staphylococcus aureus was isolated from 12 samples (20%). The highest contamination rates were found in chicken shops (31%), barbecue shops (22%), and protein shops (21%). Additionally, PCR analysis revealed that the sea gene was not present in any of the isolates. Staphylococcus aureus can easily cause outbreaks of staphylococcal food poisoning by contaminating raw chicken barbecue. Therefore, implementing quality control standards and emphasizing food safety among the public is essential, as is investigating the prevalence of enterotoxin-producing genes in Staphylococcus aureus isolates from raw barbecue chicken meat.

https://vcbr.uk.ac.ir/article_4815_6264934c1bf20ac15439666e6b7bc192.pdf